Intestinal iQ™ - The New Way to Assess Intestinal Status


FLUIDS iQ® recently launched our updated series of intestinal tests, featuring our triad of Zonulin, Diamine Oxidase\Histamine and Total IgG food sensitivity tests. We call it Intestinal iQ™ and it comes with an enhanced graphic layout of the results together with a detailed interpretation.


Why Intestinal iQ™?


FLUIDS iQ® is continually striving to provide wellness professionals with innovative and clinically useful tests. Our Zonulin test has been extremely well received, often being called a ‘game changer’ by those who use it. In combination with Total IgG for 24 or 88 foods it has proven to be an extremely useful screening tool of intestinal status. Ongoing research and clinical studies, together with numerous requests from the health care community, has led to our decision to add a third test to our series – the Diamine Oxidase (DAO) / Histamine test. 


Histamine is well known as one of the principle pro-inflammatory proteins, responsible for a myriad of allergic responses in the body, including increases in gastric acid secretion, intestinal inflammation and permeability. DAO is the main enzyme that breaks down histamine in the intestine and helps to maintain gastrointestinal balance.


Why Use Intestinal iQ™ tests?


Each of the tests in the Intestinal iQ™ series is useful in and of themselves. When combined, however, they paint a vivid portrait of an individual’s gut status that is unattainable when testing individual markers alone.
Zonulin, our test for “leaky gut”, is the foundational test in the Intestinal iQ™ series. Foods, nutrients, medications, alcohol, pesticides, toxins, viruses, bacteria, and fungus all come into intimate contact with the body through the gut. When the gut barrier becomes damaged by food allergens, chemicals, antibiotics, or infection, this important barrier function can be compromised, leading to increased intestinal permeability, or “leaky gut”.  


The Importance of testing the complete triad, by adding our DAO\H and Total IgG food sensitivity tests. 


Including an analysis of DAO/H enhances the portrait begun with the Zonulin screening test by assessing levels of allergic inflammation and the body’s ability to decrease inflammation in the gut. DAO and Histamine can tell if there are problems breaking down Histamine, or if there is inflammation causing allergic-type symptoms. If there are GI symptoms such as reflux, irritable bowel disease, chronic diarrhea, or food sensitivities, then this test profile helps in finding the underlying causes of these symptoms.


When Zonulin is used together with our DAO/H and Total IgG-24 or 88 foods panels, the combined profile gives a very clear and powerful portrait of the following:


1) The enzymatic and dysbiotic effects on GI function (Total IgG 24 or 88)
2) Inflammatory and permeability changes, both local and regional (DAO/H)
3) Intestinal permeability changes (Zonulin) 


Introducing Intestinal iQ™


FLUIDS iQ® is proud to announce the launch of Intestinal iQ™, our newest approach to the “gut”, featuring the FLUIDS iQ® “triad” of Zonulin, Diamine Oxidase\Histamine and Food Sensitivity testing.


About Intestinal iQ™

The FLUIDS iQ® series of easy to use Intestinal iQ™ wellness tests are focused on three key areas, which we call the “triad”:

• Intestinal permeability (“Leaky Gut”);
• Inflammation;
• Enzymatic and bacterial imbalances


“Leaky Gut”: FLUIDS iQ® ’s Zonulin test

FLUIDS iQ® ’s industry-leading dried blood spot Zonulin test for “Leaky Gut” is the foundation of all Intestinal iQ™ testing. “Leaky Gut” is a condition that occurs when the gut lining becomes abnormally permeable, allowing bacteria, large food derived molecules and toxins to pass through. A major controller of this gateway is Zonulin, a protein found in intestinal cells. Zonulin testing is a very useful first line screening test in beginning to paint a portrait of an individual’s gut status.


Intestinal Inflammation: FLUIDS iQ® ’s Diamine Oxidase\Histamine Test

Histamine (H), an organic compound, is involved in many types of allergic and inflammatory processes and helps regulate gut function. Diamine Oxidase (DAO) is the body’s primary enzyme for breaking down histamine. Histamine excess may result from insufficient DAO, or consumption of histamine rich foods.This second test in our Intestinal iQ™ triad, theDAO\H test, builds on the first and provides important information that standard food sensitivity tests may not reveal.


Enzymatic and Bacterial Imabalance: FLUIDS iQ® ’s Total IgG Food Sensitivity test
Food sensitivity is a result of immune reactions, with symptoms generally limited to the gut. This distinguishes it from food “allergy”, which may cause severe, life threatening, allergic reactions (anaphylaxis). FLUIDS iQ® ’s Total IgG 24 or 88 Foods Panels help determine the possible source of enzyme imbalances, such as stomach, pancreas and/or biliary system as well as bacterial imbalance (dysbiosis). When used together with the tests for “Leaky Gut” (Zonulin) and inflammation (DAO/H), they give an even clearer picture of gut function, a vital aid in determining treatment.


Intestinal iQ™ tests are all easy to use, dried blood spot tests. They are offered alone or in combination. Please call or email us to order your testing kits today!

The FLUIDS iQ® Diamine Oxidase (DAO)/Histamine Test


FLUIDS iQ® is proud to be launching a new addition to our line of dried blood spot wellness tests: The DAO/Histamine test for monitoring intestinal inflammation. Our test reports will show both the levels of DAO and Histamine as well as the their ratio, which is a quick way of highlighting potential imbalances.


Histamine is a bioactive amine that is produced in the body in response to an injury or foreign substance, which includes many foods. Its physiological effects include increasing blood supply, regulation of gastric acid and blood vessel permeability, as well as a normal response to inflammation. The highest concentrations are found in the gastrointestinal tract, skin and lungs, with smaller amounts in the heart and brain.


In healthy individuals, dietary histamine can be rapidly detoxified by DAO, the main enzyme for the metabolism of ingested histamine. The level of histamine in the blood, after eating histamine-rich foods, depends on its passage through intestinal cells, or enterocytes. DAO, located in the enterocyte cytoplasm, is important in inactivating histamine before it enters the blood system.


An impaired histamine degradation due to reduced DAO activity, and the resulting histamine excess, may create symptoms that mimic an allergic reaction. This "Histamine Intolerance (HIT)" results from a disequilibrium of accumulated histamine and the capacity for histamine degradation.


The ingestion of histamine-rich food, alcohol or drugs, that release histamine or block DAO, may provoke headaches, diarrhea, rhino and conjunctival symptoms, hypotension, asthma, arrhythmia, urticaria, pruritus, flushing, and other conditions that comprise the entity known as histamine intolerance. Symptoms can be reduced by a histamine-free diet or be eliminated by antihistamines.


Studies suggest that circulating DAO may be a marker for mucosal integrity and maturity.1 Certain medications may affect histamine balance in the body by promoting histamine release or inhibiting DAO.2


Total histamine load must be considered when evaluating histamine intolerance, and a balance, or ratio, between DAO and histamine appears to be crucial to maintaining gastrointestinal, skin and rhino-conjunctival health.


1. Komericki P, Klein G, Reider N, et al. Histamine intolerance: lack of reproducibility of single symptoms by oral provocation with histamine: a randomised, double-blind, placebo-controlled cross-over study. Wien Klin Wochenschr. 2011;123:15-20.
2. Maintz L, Novak N. Histamine and histamine intolerance. Am J Clin Nutr 2007;85:1185-1196.

FLUIDS iQ®'s New Look Zonulin Report


FLUIDS iQ® is excited to announce an update to our Zonulin reports, including an enhanced graphic layout of the results.


Why an updated report?

Analysis of accumulated data from a high volume of Zonulin samples prompted us to refine the present measurable reference range of 5-25 ng/ml to the new, revised range of 1-20 ng/ml. In addition, the depiction and explanation of the Zonulin result now has an enhanced graphic layout , as well as a more detailed result interpretation.


This update will provide the reader with a Zonulin value that is more closely related to the clinical picture and less prone to misinterpretation.


What are the Changes and their Meaning?
The “measurable (reference) range” shown in the reports up to now, has been interpreted by many as meaning the “normal range”, implying that people who have Zonulin levels within this range should have normal intestinal function and should not be showing signs and symptoms of intestinal permeability problems.


That is not the case. The “reference range” in this report is defined as the range of values derived from a normal distribution of results that encompass 95% of randomly selected individuals in a population.


In other words, this range of values includes individuals with normal intestinal function, through to those who have some form of overt or diagnosed inflammatory and/or autoimmune condition. To be sure that this definition is clearly understood, it has been written just below the Zonulin colour bar, next to the asterisk (*)


To further emphasize how the Zonulin values relate to permeability change, the horizontal bar has also been divided into three sections, each with its separate colour and word description:


     • Green for Optimal (1-6 ng/ml)

     • Yellow for Borderline (6-10 ng/ml)

     • Red for Elevated (10 to 20 ng/ml). Some individuals who are in an acute inflammatory or auto-immune state may show values well above the 20 ng/ml, in some cases reaching beyond 40-50 ng/ml.


We hope that the changes in the report, outlined above, will lead to much easier interpretation.




Click here to view a sample "new look" Zonulin report

FLUIDS iQ® launches first test for Zonulin


As part of our commitment to offering the highest standards of laboratory service products and to make available tests that are at the state of the art in biomarker science,  FLUIDS iQ®  is proud to announce the launch of North America's first commercially available test for Zonulin,  the prehaptoglobin 2 protein that is recognized as a key biomarker in determining changes in intestinal permeability.


This test is quickly becoming an important part of an assessment of what is commonly referred to as “Leaky Gut” and will allow clinicians to use objective evidence in determining the clinical state of their patients.


The test for Zonulin represents only one of a number of such biomarkers tests that FLUIDS iQ® will be launching in the near future, in order to give the clinician ever greater ammunition in the targeting of individualized diagnosis and treatment.


FLUIDS iQ® ’s Zonulin test is offered as a single test as well as part of the Zonulin-24 or Zonulin-88 tests, which combine analyses of Zonulin together with a total IgG antibody response to 24 or 88 common food types.


To learn more about FLUIDS iQ® ’s Zonulin tests and for the literature on Zonulin, click here.